Rancho El Uno (ReU), managed by Cuenca de Los Ojos (CLO), operates as a nature reserve for the conservation of the grasslands of the Chihuahuan desert and the species that inhabit it, such as the American bison, the black-tailed prairie dog and the golden eagle. ReU has functioned as a training center for university students in wildlife and grassland conservation; and has prepared people to incorporate regenerative approaches into their livestock practices. As a result of these efforts, in 2022 it was possible to carry out grazing and low-stress vaccination of the herd of 328 bison that live in the ranch.